I am working with HPLC and my problem is that I have 20 standards. I got peak for each standard (with same method) but most of the peaks are in a very dense area when I compare, for example in 24:03, 23:80, 24:72. If my extract peak stand between them I can not distinguish between them. What can I do to increase the distance between peaks?
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You should optimize the eluation gradient or change some of the eluators. Please state your method to help people to get clearer picture of the issue.
I agree with Branislav. By optimising the conditions of the eluators and sometimes even a temperature control of the column can have great impact on the peak separation. Make sure that the "properties" of the extract (solvent that is carrying the extract) is same as the external standards that you use. I would also suggest the incorporation of internal standard for both extract and calibration standards.
I agree. A gradient will help separate your peaks so that they can be identified more precisely.
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