I want to conjugate a fluorescent molecule to particles I am using.
My aim is to inject it to mice veins and detect it in the mice harvested organs.
The fluorescent molecule needs to be stable and not bleach in live tissue for few days, and needs to overcome the tissue auto-fluorescence.
I can't use fluorescent protein conjugates such as PE.
Can anyone recommend such a conjugate?
Although I am not an expert in in vivo imaging, the use of cyanine dyes (e.g. Cy5) might be an option. See here for a reference http://dx.doi.org/10.1016/j.copbio.2007.01.003
Hey Christian,
I am not imaging live animal, I wish to analyze harvested organs using fluorimeter.
I need a fluorophore to "survive" for a few days in the animals to give a detectible signal.
cy5 could work if it is stable and there is no high auto fluorescence the same wavelength
Thank you
Does anyone have any experience working with cy5 conjugates in vivo ?
You could use so called up-converting nanoparticles - i.e. luminophores, which absorb in NIR (basically 980nm) radiation and emit in Vis (e.g. narrow bands at 470nm (tulium), 540 (erbium), 650 nm(Erbium), 800 nm (thulium). They are perfectly photostable, basically non-toxic, provide background free detection (due to NIR excitation and long luminescence lifetimes, which are suitable for time rosolved techniques) etc. You can find many interesting research articles and reviews on this topic (e.g. a review of my own http://www.sciencedirect.com/science/article/pii/S1748013212001223). There are a few problems that are faced with this technology : bioconjugation and dedicated instrumentation, which is not commercially available yet. good luck
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