How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

No title

When I research the ransomware detection with adversarial examples, I extracted the dataset from the strings based on cuckoo dynamic analysis. Those datasets are based on the count of each string....

08 February 2021 7,049 3 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View

What will the voltage-current characteristic of a PV string be like with each module getting different irradiance?

Suppose, in a photovoltaic string with multiple modules (assume 10), all connected in series, each module is getting different values of irradiance uniformly throughout the module surface. Each...

02 November 2020 3,826 2 View

How to clean reverse phase C18 column?

I prepared a column (reverse phase C18) for Medium Pressure Liquid Chromatography (MPLC). I injected my crude extract 5 times & now the column has become pale yellowish. My crude extract...

01 November 2020 5,276 4 View

Is comparing genetic aberrations from NGS and Transcriptome data feasible?

I am doing an analysis with transcriptome data and comparing the same with the Genomic data. More precisely, I am looking at mRNA results of a tumor sample without having the normal tumor samples...

26 October 2020 3,793 3 View

What is the error in the following Java code?

The following Java code has a run time error: package A; public class Student { Student Osama = new Student(); public static void main(String[] Nidhal) { Student Wasfi = new...

25 October 2020 6,038 11 View

How can I clear up SPSS warning that is a result of missing values encountered when converting from Numeric to String to Numeric?

Hi SPSS Users, I've been struggling for a few days with my perfectionist personality and a warning code. What I have done works, but it triggers warnings that I'd like to get rid of if I...

04 October 2020 7,586 3 View

Can anyone recommend a method of isolating terpenes (including sesquiterpenes) from dried hemp?

Can anyone recommend a method of isolating terpenes (including sesquiterpenes) from dried hemp? The idea is to separate the cannabinoids fraction from the terpenic fraction. We will use...

29 September 2020 1,267 3 View

mRNA down-regulated but protein in Western Blot still there. Which is the most plausible explanation?

How is it possible that RT-qPCR demonstrate a down-regulation of my interested gene (from 22 to 27 ct) while WB is showing the protein expressed with the same intensity as in cells where the gene...

26 September 2020 2,860 15 View