I would appreciate help with the following. I am trying to phenotype mouse B-cells. I am looking for memory, plasma, germinal center and marginal zone b-cells. I was wondering if anyone had an established panel to use on a Fortessa or LSRII?
The question is aimed at understanding how budget implementation can contribute in the growth of private universities
17 June 2024 555 1 View
I have been trying to simulate the diffraction efficiency(y-axis) versus wavelength (x-axis) for a blazed grating in Lumerical. I built the structure as a simple blazed grating made of Aluminium....
08 February 2024 7,963 3 View
That is, Exchanging some of the Foreign Debt owed by the LDC to fund Green Projects in that country.
10 July 2023 1,477 0 View
Rather than trying to recreate the wheel, I hope someone could point me in the right direction. I am preparing a seminar-type course to guide highly capable high school students through the...
24 March 2023 1,210 3 View
I'm trying to compare several linear regression models, however many of them do not report their SEE. Is there an alternative method to calcuate the SEE when we do not have access to the original...
09 August 2022 6,218 5 View
Workplace bullying is a persistent pattern of mistreatment from others in the workplace that causes either physical or emotional harm. It can include such tactics as verbal, nonverbal,...
14 September 2021 9,617 0 View
Are there any obvious technical limitations that would make it impractical to use an antibody coating, using the same techniques used in ELISA, to capture targeted viral particles through a slow...
19 August 2021 549 4 View
Looking a for a suggestion of an undergraduate research topic in Electrical Engineering focusing more on electric power generation or power systems. Third year level.
05 October 2020 6,532 19 View
Hi all, I've been reading up on parametric survival analysis, especially on accelerated failure time models, and I am having trouble wrapping my head around the family of distributions. I am...
17 December 2019 345 4 View
Hello, I am doing an additive manufacturing simulation in ABAQUS/CAE 2017. I have two working subroutines: DFLUX and UEPACTIVATIONVOL which both do their jobs correctly in separate models. I...
25 November 2019 5,319 5 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Please go through my Abstract. I can also share a proposed Thesis Outline.
04 August 2024 2,077 0 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View