I am trying to isolate symbiodinium associated with corals. After centrifuging the coral tissue homogenate (using sea water), I inoculated zooxanthellae (Symbiodinium after being viewed under light microscope) cells into F2 medium and incubated at 25 degree celcius under light for 14 hours and at dark for 10 hours with continuous CO2 supply . But still I am not able to grow them. Please someone help me.
I have attached the link with which I have prepared the medium
http://www.nsm.buffalo.edu/Bio/burr/BURR%20Cultures/F2%20Media.pdf
1) Continous CO2 bubbling may not be necessary, may apply with long intervals. 2) Try different light intensities for culture,as a single one may not give result.Roughly find out what is the light intensity under natural conditions - apply almost the same, as light intensity plays an important part in its culture. 3) The heavy metals (Cu,Co & Zn) concentration comes to 4.2 ppm - which I think is too much. Did you check such a concentration in nature.
Thank you so much for your reply Sir.
I will make changes in light intensities and CO2 supply.
Actually, I didn't check the concentrations, but most of the studies regarding this algae says that F2 medium (Guillard's) is best. So I tried with that. Also, this algae is found inside the tissues of corals, especially in their gastrodermal cells. Hence, I didn't check.
Hi there,
A large number of Symbiodinium types don't readily grow in culture. This is especially true of many Indo-Pacific clade C types that are transmitted vertically from parent to offspring (see attached article by Krueger & Gates). Do you know what algal type (i.e. sub-clade) is in your corals? If not, what species of coral do you have and from where did you collect them?
Article Cultivating endosymbionts — Host environmental mimics suppor...
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