Is it a must to use FC block before FACS?

Is it a must to use FC block before FACS or just a preference? I am still doing antibodies titration and I would like to make it cheaper and less time consuming as possible. Thank you

21 January 2018 3,881 6 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,366 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

How to interpret different leached phases of metapelites?

Extraction of three different phases of metapelites, mostly phyllites as the Carbonate phase, Fe-Mn Phase, and Silicate phase has been obtained. After doing ICPMS, different results have evolved....

11 February 2021 5,198 10 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution?

How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution? When I am doing the experiment, I find it is difficult to accurately determine the total...

04 January 2021 2,694 1 View

Hybodontiformes - last survivors?

Hello Everyone, I just ran into the work of Boisvert et al., 2019, in which I found fig. 4.2 a bit confusing. It shows, that Hybodontiformes (pale green) survived till the Miocene, however, as...

13 December 2020 5,180 3 View

Is there any technique to reduce carbonate precentage in bone sample (bovine hydroxyapatite)?

Dear colleagues, I am a researcher working with bovine hydroxyapatite. I want to reduce the percentage of carbonate on the sample. Is there any suggested techniques for this? I am measured the...

19 November 2020 3,828 3 View

Label Propagation - Multi Atlas based 3D Image segmentation implementation. The basics?

How do i get started with the implementation of Label propagation (Multi Atlas based Image segmentation) using sparse coding? The same has been implemented in the paper Representation Learning: A...

17 November 2020 8,569 7 View

Why do I receive the sae mesage several times?

26 October 2020 409 1 View