What is the Best human T cell for transduction?

Dhouha Daassi @Dhouha-Daassi

14 March 2022 1 10K Report

what are the most stable human CD4+ T cell line for transduction?

thank you

Malcolm Nobre

Hello Dhouha Daassi

You can use HuT78 CD4+ T cell line

Culture medium: IMDM + 20% FBS + 2mM L-glutamine.

Jurkat E6.1 T cells

Culture medium: RPMI + 10% FBS +2 mM L-glutamine

Please refer to the article attached.

Article Optimization of Methods for the Genetic Modification of Human T Cells

Best.

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