Can anyone give me the mitochondrial isolation method from liver cells?
To get close Pr , look at the documents below, hope this is useful:
1-http://www.nature.com/nprot/journal/v2/n2/abs/nprot.2006.478.html
2-http://www.sciencedirect.com/science/article/pii/S0891584999002373
3- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1200198/
4- http://www.jbc.org/content/172/2/619.full.pdf+html?sid=8e3df3f5-a361-44d3-b696-3e52a3f87135
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082392
Frezza C, Cipolat S, Scorrano L (2007) Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat Protoc 2: 287–295. doi: 10.1038/nprot.2006.478
Rogers GW, Brand MD, Petrosyan S, Ashok D, Elorza AA, et al. (2011) High throughput microplate respiratory measurements using minimal quantities of isolated mitochondria. PLoS One 6: e21746. doi: 10.1371/journal.pone.0021746
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