Hi everyone, am trying to digest cellulose using bacteria. Could someone give me a technique that can be employed, to ensure that we get NCC and the hemicellulose which is waste is removed from the reaction mix.
Thank you
Leah
Hello Leah
I think TEM images of the digested cellulose is a good start to confirm the shape (thin rods). Then determining Degree of Polymerization and crystallinity (using XRD) will be a supporting evidences. Digesting cellulose will not produce hemicelluloses, it will produce oligosaccharides. You can hydrolyze with an acid to glucose and determine them using HPLC.
Hi Mohammad,
We are generally doing acid hydrolysis, but the process is inefficient, and an environmental hazard.
The process of acid recycling is tedious, so, we are researching on replacing it with controlled enzyme hydrolysis. That is where we start experiencing problems.
Hi Leah,
I think is better to using pure cellulose as you can control the reaction.
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