Fluorescence quenching
Hmm... something like,
The absorption (or energy transfer) of the fluorescence emission
(doesn't even have to be to another molecular identity, although I think it usually is)
(Tutorials on FRET provide a pretty good conceptualization)
Good luck!
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...
22 February 2021 338 9 View
Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...
18 February 2021 4,540 5 View
In the museums, the sensitive organic materials need to be displayed under 50 lux or less and aways from any UV or IR. LEDs still have a little amount of UV. What is the better option? Is it...
20 January 2021 5,965 3 View
07 January 2021 5,410 8 View
The FFPE tissues are relatively difficult to extract the RNA from so, how one can use reverse transcription based PCR
10 December 2020 4,398 6 View
when i did time history anlysis by using sap 2000 , the program showed the first two step only at .02 and .04 . can anyone help me to solve this problem?
27 November 2020 3,810 2 View
I want to know how can I do an analytical solution for stresses distribution due to tunnel excavation in an expansive soil to compare with a numerical simulation. and which method I can use?
22 September 2020 7,602 2 View
Can any one help me in meshing in ABAQUS, please?
09 September 2020 7,957 7 View
Chlorine, a component of PVC polymers, acts like a halogen-based flame retardant agent in the event of fire.
07 September 2020 5,450 6 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View