Most recent, reliable, optimized methods to extract both RNA and protein from flash-frozen brain tissue?

I have flash-frozen brains and am looking to extract RNA for RNA-seq, and protein for western blotting. I've seen other questions related to this...

10 December 2020 6,294 0 View

Cyclic Voltammetry: Small peaks near 0V in electrolyte solution without analyte?

CV setup is glassy carbon WE, Pt wire CE, and Ag/AgNO3 RE (no salt bridge). My solvent is DMSO with tetraethylammonium tetrafluoroborate (TEAFB) as the electrolyte. The reference electrode...

09 October 2019 330 4 View

What factors could lead to these banding patterns following basic restriction cloning?

I have been attempting what should be a basic subclone procedure for several weeks now to no avail. After performing a ligation of the vector and single insert (both double-digest DraIII/SbfI),...

07 August 2019 743 2 View

What might cause the ~160bp peak in this library prepared using FFPE extracted RNA and Takara SMARTer kit?

16 June 2019 1,703 2 View

Can anyone help in testing for endogeneity and other diagnostics for a mixed effect model using Stata? Can I make private contact with anyone?

I am using unbalanced panel data and the mixed effect model gives better results. I would need assistance on how to test for endogeneity and run appropriate diagnostics using Stata 12. Thanks

05 June 2018 8,247 0 View

Why do I get similar RNA concentrations from different amounts of cells eluted in the same amount of liquid?

I'm using the Ambion RNAqueous Micro Kit to extract RNA from cells in different pathways. I use laser capture microdissection to extract the cells. From one pathway, I normally collect 8-50 cells....

07 March 2017 8,379 4 View

Is there a photo-bleachable and cell permeable nucleic acid stain for live imaging experiments?

I want to ablate a subset of cells in live mammalian tissue explants and watch how the surrounding cells develop. I am looking for a vital dye to label nuclei that is also photo-bleachable,...

02 February 2015 2,210 2 View

Does anyone know good electrochemical references for organic solvents other than Ferrocene?

Just looking for another electrochemical system to use as an internal standard for an organic system of mine. Ferrocene oxidation happens to occur exactly at the same potential (well within 0.05V)...

02 June 2013 4,530 17 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View