Fresh specimen can be easily processed for SEM, but not samples which have been fixed either in alcohol or carnoys fixative
Dear Ummer, you can try to remove the Carnoy of the helminthes, wash in PBS and post-fixed in 1% osmium tetroxide by 2 h. There upon, the excess rinse in PBS again (or water), dehydrated in increasing acetone series starting at 30% acetone, 40%, 50%, 70%, 90% and 100%, dry in the critical point of CO 2, and after, to do the metallization. I have processed helminths fixed in 10% formaldehyde (preserved inside hosts in museum collections), or fixed in A.F.A. or Glutaraldehyde and I have obtained good information.
Dear Ummer
samples fixed in AFA (92 parts ethanol 70%, 5 parts of formalin + 3 parts of acetic acid) and preserved in 70% alcohol can also be easily processed for SEM using the technique suggested by Dr. Jeannie Santos.
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