Which solvent mixture should be used in Thin layer chromatography as a mobile phase?

I am going to do analysis of antioxidant activity of some medicinal plants, for that I should go through solvent extraction. I want to ask that should I use the same solvents for thin layer...

09 October 2015 5,713 7 View

Is it neccesary to perform thin layer chromatography after plant extraction?

07 August 2015 4,902 16 View

What is the most efficient method to separate lignin degrading enzymes such as Laccase, Mangnese peroxidase, Lignin peroxidase?

08 September 2014 3,736 6 View

Is there any need to employ polar solvents for plant extractions?

06 July 2014 2,471 15 View

How can I find out what enzyme inhibitors are present in medicinal plants such as Cassia spectabilis and Caesalpinia pulcherrima?

I am doing work on some medicinal plants (mentioned above). I want to know which enzyme inhibitors are present in such plants as I have to select specific enzymes for enzyme inhibition assays. The...

04 May 2014 1,952 3 View

How can I find out the antioxidant activity of a medicinal herb? What is the suggested or recommended method?

I am research associate working on a project about herbal medicine.

10 November 2013 8,344 12 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View