Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How can I find the effect of different parameters like (Reynolds number, Hartman number, volume friction etc.) in COMSOL?

Here I am facing this problem. I don't find any effect of this type of parameters. I am a beginner user of COMSOL.

23 February 2021 9,086 3 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

Can fractal dimension of thin film surfaces affects the electrons energy states?

As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?

19 February 2021 5,509 3 View

Can you slightly adapt a measuring scale without damaging it's validity?

I am trying to research Agency in dreams, and seeing as there is isn't a specific scale to measure dream agency, I thought about adapting a known agency scale. For example the item "I Feel in...

09 January 2021 3,657 3 View

What is the new in new year 2021?

2020is finished and was very hard for millions now how to welcome new year.now what is our wishes in new year e year 2020 has been an unpredictable ride through the pandemic, protests and many...

01 January 2021 1,200 5 View

Has anybody used computational linguistics-based, AI-assisted analytics for their research like the covid-19 study below?

16 November 2020 8,316 3 View

Is dreaming a synapomorphy?

I was wondering if, in evolutionary biology terms, dreaming is a synapomorphy for a certain delimitated group of animals. If you can point me out a textbook or papers to read more about it, I will...

27 July 2020 4,601 5 View

What are your dreams for ICM?

Congratulations & Good Wishes ICM Celebrates 3rd Anniversary If there is ever a corporate history written on ICM, it will start with you and end with you. You are not just another amazing...

24 July 2020 6,454 3 View

How to isolate full lenght of SbBADH1 and SbBADH2 ?

Hello, It was easy to get the full length of SbBDAH1 (1520 bp) & SbBADH2 (1517 bp) from cDNA using dream taq from thermo scientific, although there were non specific targets and primer...

27 February 2020 5,848 6 View

Which tools do you use for Systems Engineering?

Using a perfect toolchain for modeling every aspect of a system is possibly a dream. But perhaps one of you can recommend a tool, that supports most systems engineering tasks.

06 February 2020 2,909 3 View

How to isolate full lenght of SbBADH1 and SbBADH2 ?

Hello, It was easy to get the full length of SbBDAH1 (1520 bp) & SbBADH2 (1517 bp) from cDNA using dream taq from thermo scientific, although there were non specific targets and primer...

01 February 2020 3,386 3 View

How to be strong to fulfil your dream in life?

to be strong and happy is very important ,let concentrate on these factors. 1-faith and let others feel that you are frank band sincere and never lie. 2-take care of your health . 3-take care of...

31 December 2019 9,341 2 View

Should'nt a female(doctor) researcher from a developing country ever dream of doing Phd?

Waiting to be selected in a fully funded Phd position in physiology/neuroscience/psychology.When will my dream come true?

10 November 2019 3,731 3 View