I would also like to know if DACIA is comparable in activity to another thiol protease probe N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM). Any suggestion will be of great help.
I have been attempting Site Directed Mutagenesis on my gene of interest cloned in the vector pET28A+. I began with two mutations, did PCR and transformed the PCR products into BL21DE3 cells. After...
07 August 2018 3,911 7 View
I have been growing quite large protein crystals after several rounds of standardization. However good the crystals may look, I am getting very faint diffraction spots from them, that too, around...
06 July 2018 1,258 7 View
If a protein has at least 15 free Cysteine residues, is getting expressed but is difficult to purify; how can the purification procedure be optimized to get augmented amounts of stable protein?
11 December 2017 1,414 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...
20 February 2021 1,291 3 View
I'm using blast furnace slag for stabilizing subgrade soil.
17 February 2021 7,333 3 View
Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...
16 February 2021 453 2 View