I would also like to know if DACIA is comparable in activity to another thiol protease probe N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM). Any suggestion will be of great help.
I have been attempting Site Directed Mutagenesis on my gene of interest cloned in the vector pET28A+. I began with two mutations, did PCR and transformed the PCR products into BL21DE3 cells. After...
07 August 2018 3,975 7 View
I have been growing quite large protein crystals after several rounds of standardization. However good the crystals may look, I am getting very faint diffraction spots from them, that too, around...
06 July 2018 1,364 7 View
If a protein has at least 15 free Cysteine residues, is getting expressed but is difficult to purify; how can the purification procedure be optimized to get augmented amounts of stable protein?
11 December 2017 1,476 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Bioinformatics tools like peptide cutter
15 July 2024 6,453 1 View
what all details we will get from fluorescence life time data
10 July 2024 505 1 View