Subhoja Chakraborty

4 Questions 6 Answers 0 Followers

Questions related from Subhoja Chakraborty

How do I design colony PCR primers?

I have been attempting Site Directed Mutagenesis on my gene of interest cloned in the vector pET28A+. I began with two mutations, did PCR and transformed the PCR products into BL21DE3 cells. After...

08 August 2018 3,947 7 View

How to improve the diffraction quality of protein crystals?

I have been growing quite large protein crystals after several rounds of standardization. However good the crystals may look, I am getting very faint diffraction spots from them, that too, around...

07 July 2018 1,322 7 View

How to optimize the purification procedure of a protein that has many free Cysteines in its structure?

If a protein has at least 15 free Cysteine residues, is getting expressed but is difficult to purify; how can the purification procedure be optimized to get augmented amounts of stable protein?

12 December 2017 1,449 1 View

Can anybody provide any information on the availability of the fluorescent probe N-(7-Dimethylamino-4-methylcoumarin-3-yl)iodoacetamide/DACIA?

I would also like to know if DACIA is comparable in activity to another thiol protease probe N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM). Any suggestion will be of great...

10 October 2017 4,291 0 View