We isolate this fungus from black bean aphid. By our little knowledge we suspect this is Beauveria sp!
Hello Dear
That is very similar to Beauveris. But I have not systematic special. Best regards
Thanks dear Dr. Masoud Latifian. It will be very helpful if you provide me some systematic reference for identification of beauveris or /microscopic images.
Dear Dr. Laith Al-Ani there is any simple distinguished feature between anamorph and teleomorph stages?
The teleomorph has fruiting bodies, like mushrooms. There are so many Beauveria species nowadays, that it's best to have your fungus identified by DNA analysis.
Dear Dr. @Christiaan Kooyman , in our lab we identified fungus by D2 LSU rDNA sequencing ; is this enough for differentiate between teleomorph & anamorph? or need any specific primer
Dear Jubair. Teleomorph and anamorph are genetically similar and can therefore not be differentiated by any type of sequencing. The teleomorph is recognized by the formation of fruiting bodies inside which ascospores are formed. The hyphae of teleomorphs typically have cells with two nuclei (dikaryon). Fusion of nuclei (karyogamy) occurs in the asci immediately followed by meiosis. The conditions that trigger the transition between the two states, are still poorly understood, including what happens at the chromosome level.
By the way, did your sequencing allow you to identify the species of Beauveria? If so, which species is it?
Dear Dr. Christiaan Kooyman we used FAST MicroSEQ D2 LSU rDNA Fungal Identification Kit.
Sorry Dr. we don't sequence it yet. we need some confirmation test before sequencing.
It is Beauveria Bassiana. I have already isolated from the desert locust
Dear all
I attached few more pictures of our Beauveria suspected isolate. Hope it will help you all to identify.
Hi Jubair. Even though so many people claim that this is Beauveria bassiana, I urge you to have your isolate sequenced to make sure which of the about 50 species of Beauveria this is. Things are not as simple any more as 20 years ago.
Dear Jubair Al-Rashid and Christiaan Kooyman
https://en.wikipedia.org/wiki/Molecular_Koch%27s_postulates
Biotech tools not to forget about traditional way even the new koch's postulates (1890) called Molecular Koch's postulates
Molecular Koch's postulates are a set of experimental criteria that must be satisfied to show that a gene found in a pathogenic microorganism encodes a product that contributes to the disease caused by the pathogen. Genes that satisfy molecular Koch's postulates are often referred to as virulence factors. The postulates were formulated by the microbiologist Stanley Falkow in 1988 and are based on Koch's postulates.[1]
The postulates as originally described by Dr. Falkow are as follows:
1. "The phenotype or property under investigation should be associated with pathogenic members of a genus or pathogenic strains of a species." Additionally, the gene in question should be found in all pathogenic strains of the genus or species but be absent from nonpathogenic strains[citation needed].
2. "Specific inactivation of the gene(s) associated with the suspected virulence trait should lead to a measurable loss in pathogenicity or virulence." Virulence of the microorganism with the inactivated gene must be less than that of the unaltered microorganism in an appropriate animal model.
3. "Reversion or allelic replacement of the mutated gene should lead to restoration of pathogenicity." In other words, reintroduction of the gene into the microbe should restore virulence in the animal model.
For many pathogenic microorganisms, it is not currently possible to apply molecular Koch's postulates to a gene in question. Testing a candidate virulence gene requires a relevant animal model of the disease being examined and the ability to genetically manipulate the microorganism that causes the disease. Suitable animal models are lacking for many important human diseases. Additionally, many pathogens cannot be manipulated genetically.
References[edit]
1. Jump up ^ Falkow S (1988). "Molecular Koch's postulates applied to microbial pathogenicity." Rev Infect Dis 10(suppl 2):S274-S276.
Regards
Houda
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