Can we use Aluminum sheet as a substrate for thin film and have surface area calculated from BET?

I am to calculate surface area of a thin-film using BET and use the same thin-film for electrochemical analysis. I have chosen aluminum sheets, will there be any issues with BET??

27 September 2020 8,456 4 View

Where can I get sea salinity, surface sea temperature, chlorophyll content of bay of bengal for developing machine learning model?

I have some data in the form of maps but I prefer data in reading format and data corresponding to all gps locations.

04 August 2020 3,882 1 View

Virus Methods: Calculationg Virus titer and deciding which titer would be better for my experiment?

I was looking for this chapter titled 'Virus Methods' in a book 'Baculovirus expression vectors : A laboratory manual by David R. O'Reilly, Lois K. Miller, Verne A'. However, I do not have access...

27 March 2017 9,068 11 View

Why do MCF7 spheroids not get formed using the low-adhesion well-plate technique?

I have been trying to culture MCF7 spheroids by seeding 5000 cells in DMEM, in a low-adhesion 96 well-plate (Corning) and then centrifuging at 1000g for 10 mins. However, I am only able to get...

06 March 2017 3,510 4 View

What is the best protocol for subcellular fractionation of Gram-positive bacteria?

11 January 2017 9,996 1 View

Why does the HIV-1 expansion not work after use of repeatedly expanded HIV-1 of a lab adapted strain as the initial stock?

Since I am working on first such project, it would be great if someone can explain me about this: While expansion of HIV-1 viral stocks of lab adapted strains, which is a standard procedure of...

01 January 1970 8,771 3 View

I want to make a sacrificial layer which is unaffected by resist removers. What is the best way to do it?

I'm trying to make a sacrificial layer on a glass substrate. I'll deposit gold on top of it and do lithography on it to write some structures. I have other plans with it afterwards. I'm facing...

01 January 1970 7,270 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View