Can anybody help me to confirm the identification of this seaweed species?

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Can anybody suggest a correct identification for this marine gastropod species?

Collected from the rocky intertidal habitat, Central west coast of India.

30 July 2022 5,450 3 View

Does autophagy inhibition lead to reduction in lipid accumulationin hepatocytes grown in presence of free fatty acids?

I wish to study the effect of autophagy inhibition on lipid accumulation in HepG2 cells when treated with free fatty acids (palmitic acid and oleic acid). I have observed that HepG2 cells when...

28 February 2022 9,686 1 View

Can we use Aluminum sheet as a substrate for thin film and have surface area calculated from BET?

I am to calculate surface area of a thin-film using BET and use the same thin-film for electrochemical analysis. I have chosen aluminum sheets, will there be any issues with BET??

27 September 2020 8,608 4 View

Where can I get sea salinity, surface sea temperature, chlorophyll content of bay of bengal for developing machine learning model?

I have some data in the form of maps but I prefer data in reading format and data corresponding to all gps locations.

04 August 2020 4,013 1 View

Can I use human whole chromosome painting probes for X and Y chromosomes in cattle??

Can whole chromosome painting probes of human give results in cattle semen samples??

26 August 2019 831 2 View

Can i use human X and Y chromosomes centromeric probes for the cattle semen samples???

Iam having X and Y centromeric probes of humans and i want to use those probes on bull semen samples for the diagnosis of aneuploidy condition. Can i do that or not???

02 August 2019 5,319 0 View

Hello sir, It's great, you are working on Recycling project, in coming future, it'll have a peak demand. However, may I know more details of project?

No as such details

09 August 2017 1,807 1 View

Lower bound for complexity of language emptiness checking in visibly pushdown automata?

What is the best known lower bound for the complexity of language emptiness checking in visibly pushdown automata? Alur in [1] gave O(n^3) as upper bound. [1] Rajeev Alur and P. Madhusudan. 2004....

18 April 2017 8,566 0 View

Virus Methods: Calculationg Virus titer and deciding which titer would be better for my experiment?

I was looking for this chapter titled 'Virus Methods' in a book 'Baculovirus expression vectors : A laboratory manual by David R. O'Reilly, Lois K. Miller, Verne A'. However, I do not have access...

27 March 2017 9,251 11 View

Why do MCF7 spheroids not get formed using the low-adhesion well-plate technique?

I have been trying to culture MCF7 spheroids by seeding 5000 cells in DMEM, in a low-adhesion 96 well-plate (Corning) and then centrifuging at 1000g for 10 mins. However, I am only able to get...

06 March 2017 3,671 4 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Hello researchers, how can one get the optical density of a filamentous algae?

i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...

30 July 2024 5,985 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View