Murine protein expressed in HEK293 cells used for aspirations in mouse model ?

I am planning to express a murine protein X with a Myc tag in HEK293F cell line - purify the protein - check the purity of the protein - use that protein - X with Myc tag to aspirate mouse. Thus,...

12 February 2020 4,483 1 View

How to get an Asymptote from multiple coordinates obtained from Machine Learning ?

I want know about an algorithm or formula to find the asymptote from coordinates obtained from Machine Learning. Like the ML will always give points precise & closer to the Asymptote if I ran it,...

11 October 2019 1,025 2 View

I have completed my bachelor's in Electrical Engineering and I am looking forward to start learning more about smart grid .From where should I start.?

I want to learn about smart grid and simulating a smart grid network and also the data analytics used in smart grid network .

23 May 2018 6,165 3 View

Has Any One Tried 365nm Fixed Wavelength LED 10 Watts?

29 September 2016 8,339 3 View

Does anyone have experience working with Injectable Cyanocobalamin in rats?

15 September 2014 1,045 4 View

When I tried to precipitate my protein with acetone or TCA, it did not precipitate. But when I mix it with water it precipitated - any thoughts?

My protein is acid extracted. I need to know the mechanism behind it.

29 May 2014 6,787 3 View

Free software to conduct an online quiz?

From the last few months, I am trying to generate an online Quiz for my student, I have prepared some of them also, but find two difficulties: 1. Some students can't open the exam in their...

24 December 2013 2,307 3 View

Shortcut key to merge some cells in table & to split the same!

Can anybody suggest the shortcut keys for Merging & splitting the cell in MS word & MS Excel?

05 October 2013 9,215 13 View

What is the ideal dilution for using the Primary and Secondary Antibody Concentration for 6x HIS tag : Protein?

The 1:2000 for primary and 1:20000 for secondary gives a very dark background .

22 May 2013 4,813 3 View

What if the restriction digest pattern of plasmid midiprep varies from the same plasmid's miniprep?

I normally inoculate 5 - 7 colonies from selective antibiotic LB plate after transformation in the liquid LB with the same concentration of the same antibiotic. The next day, I do plasmid miniprep...

27 February 2013 4,896 12 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Hello .Ineed a researchs about systolic heart failure any one can help me ?

Am a master student .I need an information about this case to write my thesis?

09 February 2021 9,706 2 View

How the annotations of MIT-BIH Arrhythmia Database can be viewed in MATLAB?

Basically i want all classes of arrhythmia in single .mat file with proper labels. What should i do?

09 February 2021 8,062 2 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

Is there any method I can use to isolate single cells (i.e cardiomyocytes and non-cardiomyocytes) from snap from rat heart tissue?

I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...

19 January 2021 4,044 3 View

Are COVID 19 vaccines reliable for people with medical conditions, like those with respiratory issues, diabetes, heart problems, etc.?

Are COVID 19 vaccines reliable for people with medical conditions, like those with respiratory issues, diabetes, heart problems, etc.?

22 December 2020 5,948 7 View

Help determining the most important covariable?

Hello everyone, I'm rather new to data science as a whole and I've come accross this issue. I have a dataset in which patients are granted a score based on certain variables (blood pressure,...

16 December 2020 9,361 3 View

What part of heart ?

Hello You can see a transversal slice of a mouse heart in microscopie with blue coloration. My question : what part of the heart is it ? how i can recognize difference between histology of...

01 December 2020 1,000 3 View

Anyone with experience conducting proximity ligation assay (PLA) in mouse heart tissue ?

I was wondering if anyone had any experience doing proximity ligation assays (PLA) using mouse whole heart (ventricular) tissue (in any state)?

29 November 2020 9,103 1 View