How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

Is this right way to get Modulation Transfer Function(MTF)?

Hi I'm trying to make a python script to get MTF curve for the input image Here is my algorithm to get MTF. 1. get Image 2. select ROI(High contrast region) 3. extract a center line out of image...

15 August 2020 6,646 3 View

How to correctly indicate a zinc finger in a protein for molecular dynamics?

I am preparing a protein that has a Zn finger for molecular dynamics. To indicate the finger of Zn a patch is placed in which the deprotonation of the cysteines is necessary so that the Zn ion can...

05 March 2020 1,425 3 View

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28 February 2020 1,633 2 View

How can I use with ECMWF wind and pressure in MIKE 21 HD modelling and having sinusoidal curve of surface elevation?

I have ECMWF ERA-5 wind and pressure data in NetCDF file, and i was able to convert the data to dsf2 file for use in MIKE 21 HD model. The problem that the results of the simulation (surface...

19 November 2019 3,850 3 View

How do I calculate solar absorbance and thermal emittance of absorber coating from reflectance spectrum using standard AM 1.5 and blackbody radiation?

I have a reflectance data of my solar absorber coating from UV-Vis, I want to calculate the solar absorbance of my coating using standard reference spectrum AM 1.5 and thermal emittance of thesame...

15 November 2019 225 3 View

How can Iimprove the resolution on a GC?

When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just...

22 August 2019 4,050 6 View

What is the solution to the error while running MaxQuant ?

Dear all, I use label-free QTOF technique (Bruker) in my project. I got a good result at the first time I analyzed .d folder using MaxQuant version 1.6.5. However, in the last 2 months, error...

18 August 2019 5,009 6 View

Can anyone help me to find a rapid sensitive assay for quantitative methylation analysis ?

Hello all, in my research I need to do quantitative methylation analysis on 3 candidate genes and have to do it in only 4 months, can you please help to find a fast but sensitive method for that?...

19 April 2019 7,728 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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01 March 2021 3,905 5 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View