Dear Maliha,
The following recent review article entitled " Methods for in vitro evaluating antimicrobial activity: A review" by Balouiri et al. published in Journal of Pharmaceutical Analysis Volume 6, Issue 2, April 2016, Pages 71–79 describes all available methods (ne and old) for the determination of antifungal and antibacterial activities:
Abstract
In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.
http://www.sciencedirect.com/science/article/pii/S2095177915300150
Hoping this will be helpful,
Rafik
Dear Maliha
See the research paper for complete solution of question. Kalpesh. B. Ishnava, K.H. Chauhan and C.A. Bhatt (2011). Screening of antifungal activity of various plant leaves extracts from Indian plants. Archives of Phytopathology and Plant Protection. Vol.45 (21): 1-9.
I hope this article useful for your research.
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