Optimizing annealing temperature for primers?

Hello everyone! I got a similar query! I have calculated Tm for my primers and those were in the range of 58-62. However, when I received from the company, those were in 53-58 range. And now the...

25 August 2020 5,220 8 View

What are easy, simple and reliable methods to systematically study, measure and analyze endothelial function?

I am interested in studying all the physiological, neural, endocrine mechanisms associated with different breathing exercises suggested by traditional pranayama - such as ujjayi, bhramari, nadi...

09 August 2020 5,102 4 View

What is a good book on matrix theory (linear algebra) as applied to the study of graphs?

I am interested in studying the anatomical and functional connectivity of human brain using graphs, and their evolution in time. This can be used to distinguish different states in which the brain...

05 April 2020 5,693 7 View

Why isn't voltage adding up in super-capacitor model of MATLAB/SIMULINK?

I have designed a synchronous buck-boost converter for the supercapacitor. I've set the rated voltage to 32v and the number of series capacitors to 1. However, on the input side, the voltage...

11 March 2020 8,841 5 View

How to model/simulate Li ion battery using MATLAB/Simulink?

I'm using the Powertrainblockset toolbox and would like to estimate SOC, voltage, and current. The reference section on Matlab documentation doesn't seem to give me any idea on how to go about it....

01 February 2020 3,570 2 View

What is the complete anatomy and physiology related to the autonomic control of heart rate, blood pressure & respiration and interrelationships?

The phenomenon of respiratory sinus arrhythmia is well known. But, I don't know (I am an engineer) the exact receptors, feedback mechanisms, cerebral control centres, etc. involved in this...

02 February 2019 7,800 6 View

How to extract a compound from adipose tissue for LCMS-MS analysis?

I need to evaluate how much of an administered compound is present in mice adipose tissue. Does anyone have a protocol or paper that they can recommend on how to extract a compound from adipose...

03 October 2017 6,910 3 View

How do you effectively inject cells into SCID mice via tail vein injection to develop a CML model?

I am trying to establish a cell line based xenograft model of CML in SCID mice. I tried to inject 5x10^5 K562 cells (in saline for injection) but there was considerable difficulty in injecting the...

13 July 2017 1,371 3 View

Can paraffin embedded sections be used for autoradiography with PET tracers?

We have only used frozen sections for autoradiograpy with PET tracers so far. One of our collaborators is interested in testing some paraffin embedded sections. Can they be used? Has someone...

12 July 2016 3,413 8 View

How to design two wheelers for better road-holding without compromising ride quality?

26 May 2016 1,521 4 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View

How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View