What are the important factors for effective labeling? What is the most effective method : labeling after annealing or annealing after labeling?
It is a kind of Hetero Diels Alder reaction follows concerted pathway as obtained from experimental observation and results.
10 November 2014 4,790 5 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...
07 July 2024 5,614 5 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....
19 June 2024 2,680 1 View