Conducting a survey to understand the response to work from home during the covid-19 lockdown?

Greetings, We are conducting a survey on work from home and its impact, please fill the questionnaire and contribute to the growth of academic understanding of the concepts and for policy...

03 May 2020 3,758 22 View

What are recommended temperature conditions for purifying his tagged proteins under denaturing conditions(GuHcl)?

Hello everyone, I'm trying to purify his-tagged protein under denaturing conditions using Guanidine Hydrochloride (Protocol from GE Healthcare-attached below). Though I'm getting my protein in...

10 December 2019 8,654 3 View

How to access stress/ strain at integration points in a umat code?

Im trying to code a damage model for beam elements, for which I know the strain at rupture. I was wondering if I could access the strain at the center of the beam to start and progress damage. As...

28 May 2019 9,755 1 View

How much is 1 volume of 70% ethanol?

I need to isolate RNA from the tissue sample. The procedure is given like "add 1 volume of 70% ethanol to the lysate, and mix well by pipetting". How much ethanol should I add?

19 November 2017 8,272 5 View

Bacterial intracellular synthesis of nanoparticles,Could anyone suggest any standard bacteria(preferably gram negative bacteria)for NP synthesis?

Hi, this is part of designing a practicles for students, could anyone suggest any bacteria mediated intracellular synthesise of any nanoparticle (10-50nm) and that gives highly repeatable...

13 December 2016 3,835 4 View

What is the easiest , quickest and cheapest method to separate water from dilute glycerol solution?

I have an idea of collecting water from air. I mean the water vapor which is present in air.For this we are using glycerol as it's hygroscopic . We need some easy, cheap method to separate water...

11 December 2016 9,665 11 View

What is the method for separate out the mixture of Ethyl acetate and tetrahydrofuran ?

30 June 2016 9,968 9 View

What are the possible causes of piston or gudgeon pin failure?

Piston pin is subjected to both shear and bending loads. I need some clarity in this scenario to complete my paper. The system runs at a wide range of RPMs , so fatigue also may be one of the...

04 March 2015 4,858 4 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

22 December 2014 2,981 7 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

17 July 2014 5,372 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View