Can any one help me to acces this article: Larvicidal activity of lansiumamide B from the seeds of Clausena lansium against Aedes albopictus ?

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Рreparetion primary culture of adipocytes and mesenchymal-stromal cells from human

I am currently studying mesenchymal-stromal cells from adipose tissue. Please share how you prepare primary cultures of adipocytes and mesenchymal-stromal cells from human adipose tissue. Also...

01 January 1970 2,642 0 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,300 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,667 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,143 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View