I work with the infra patellar fat pad (IFP) we believe that biomechanical loading induced a pro-fibrotic remodeling of the IFP. I am isolating cells from the IFP and analyzing them with FACs for fibroblast and other immune cells, but I am not sure if CD140alpha (PDGFR-α) is the best marker for fibroblasts. CD140a can also stain for pre-adipocytes and pre-fibroblast. may be a combination with this ab with an other would be ideal?
Thank you