Check the light source and filters on your microscope. 

Here is a nice tool which you can use

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

For live imaging there shouldn't be a problem to use your FACS antibodies. For fixed or cryogenic-sections however you might need to change some of the clones. It's a matter of trying.  Good luck

why not ?

as long as you know what your antibody is doing and as long as you properly fluorescently labelled it...

One thing to consider is that the fluorophores best suited to flow vs. imaging are not always the same.  For instance, in flow you want very bright dyes that may not be very photostable (e.g. phycobilins (APC and PE)), whereas in imaging applications photostability becomes very important (e.g. rhodamine/fluorescein and their derivatives).  Also, it is common in flow to use antibodies directly conjugated to fluorophores (because flow is very sensitive and the preparation is simpler), whereas in imaging applications you typically want to use an unlabeled primary antibody with a fluorescent secondary antibody to amplify the signal. 

if they are commercial they will tell you FC and IF or only FC.

In addition to lamp, filters and fluorochrome exitation emission spectra, you need to considerate antigen density, as Mcconnell mentioned, 2 antibodies are needed to amplify signal most of the time.

Normally, an antibody that works on Flow would be expected to work in immunofluorescence. In fact we have done some experiments where we took a portion of FACS-stained cells and immobilized them on coverslips ( Shi-fix) and then imaged them under IF microscope. So essentially, we combined immunofluorescence and flow cytometry, getting two different data formats from one single experiment. I have covered this approach in the following post on our company website:

http://www.shikharbiotech.com/2017/11/08/one-experiment-two-analyses/

Hope that helps still !

Regards,

Ravindra

yes of course, the only points are:

1) quality of antibodies if you change the protocol for the microscopy

2) check the fluorophores in relation to the microscope you have, but in general that is more or less all the same lasers and filters, depending on what systems you have...

@all.... Thanks for the contribution. I will try out similar approach.

It looka like it didn't work...and its my first try though.....i don't know ill try to work on it....i did it just out of curiosity