sample was kept in absolute ethanol, if i can use Proteinase K deal with this sample?
Are you trying to extract DNA from the sample? If so, you should have no problem. We routinely extract DNA from fish fin clips that are stored in absolute ethanol and we don't see any problem with Proteinase K activity.
03 March 2021 8,272 1 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....
03 March 2021 506 3 View
Anyone can recommend any books on such topics? Thanks in advance.
02 March 2021 8,457 2 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?
26 February 2021 1,760 8 View
I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....
24 February 2021 4,428 3 View
I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks
24 February 2021 5,992 4 View
Recently I face some problems with the mathematical ( calculation) on the reference governor and command governor, so I need some help from you all to get which platform or journal can let me...
23 February 2021 7,746 2 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...
28 February 2021 2,955 2 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...
26 February 2021 6,905 10 View
I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...
23 February 2021 7,868 2 View
I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.
22 February 2021 9,246 1 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View