Hi,
Will it be possible to visualise exosome (50-150 nm) uptake / secretion by a TrimScope two-photon laser scanning microscopy (2PM) system (LaVision Biotec, Germany) equipped with an Olympus BX50WI fluorescence microscope and a water immersion objective (20×, NA 0.95; Olympus)?
Dear Ali, from a purist resolution approach this will be challenging and depends on where in the sample (depth/density/labelling density and signal strength) you would like to make your observation. In principle the achievable resolution with 2P excitation is lower than with a 1P approach due to the longer wavelength used and the values you state (50-150nm) are below what you would expect to easily resolve. Could you provide me some more information on the Fluorophore you are using, the wavelength set for excitation and the temporal resolution you would like to achieve? There is a convenient APP called Microscope Resolution from Andrew Barlow which allows you to get a good idea of the numbers. You may also have a look on deconvolution. Depending on the images you are able to achieve already this may be sufficient; this is for sure worth a try and may be the shortest way to the desired results. And for your information: on December 12th 2017 I will be in Bern at the MIC Symposium and would be happy to discuss directly. Best Regards, Florian
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