What will happen if a sample is send in glycerol saline 50% concentration. Why cant it be used for bacterial isolation.
It can be used......
Yes sure you can use it.
Sure you can use it.
Addition of 20-50% glycerol has proved to be favorable for survival ; at 50% glycerol, the suspension has a semi-solid condition, and samples can easily be removed without thawing the frozen culture
Yes, you can use
Hi, We are using published primers for PCR amplification of HN gene of mesogenic NDV (Forward A TGG ACA GCG CAG TTA GCC AAG & Reverse GGT AGC CCA GTT AAT TTC CA)- 2090bp product size. We use...
05 June 2017 8,764 6 View
While running the secreted protein in methanol metabolism by my Pichia pastoris GS115 clone. I am getting two band one is higher (around 50 KDa) than the desired size (41 Kda by prokaryotic...
02 March 2017 5,245 8 View
See above.
08 September 2014 6,003 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View