K. Karthik

4 Questions 12 Answers 0 Followers

Questions related from K. Karthik

Smearing in PCR- HN protein gene of mesogenic Newcastle disease virus (from field isolates )

Hi, We are using published primers for PCR amplification of HN gene of mesogenic NDV (Forward A TGG ACA GCG CAG TTA GCC AAG & Reverse GGT AGC CCA GTT AAT TTC CA)- 2090bp product size. We use...

06 June 2017 8,742 6 View

Yeast expressed protein confirmation- Two bands in SDS PAGE- one desired & other lower Kda- What may be the reason?

While running the secreted protein in methanol metabolism by my Pichia pastoris GS115 clone. I am getting two band one is higher (around 50 KDa) than the desired size (41 Kda by prokaryotic...

03 March 2017 5,225 8 View

Can a sample preserved in 50% glycerol saline be used for isolation of bacteria?

What will happen if a sample is send in glycerol saline 50% concentration. Why cant it be used for bacterial isolation.

09 September 2016 3,587 5 View

How to make poychrome methylene blue for staining Bacillus anthracis?

See above.

09 September 2014 5,972 5 View