Calorimetric LAMP technique ?

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Hossein Teimouri

If your positive control is without problems, you should set the reaction temperature and time. Try temperatures like 63 and 67. Reaction time is also important.

Anupama Karanth

Have you checked them by running in the gel. did you observe any laddered pattern band?

Did you check the DNA concentration before the LAMP assay?

If you are getting amplication then try performing with different DNA concentration and different concentration of your dye. try good quality dye