Hi all, how can I calculate the exact value of sugar unit in oligosaccharides (N-glycans)to determine the structurs. Thanks in advance
Dear Hussein,
Liquid Chromatography with Mass Spectrometric detection (LC-MS) is commonly used method to define the carbohydrate content of a an oligosaccharides.
For similar analysis and more details on the protocol to be used, you may see the publications contained in the following links:
http://sciex.com/therapeutic-protein-characterization/carbohydrate-labeling-and-analysis
http://biopharmaspec.com/services/carbohydrate-characterisation/
Hoping this will be helpful,
Rafik
thanks Mr Rafik
Hello all, I am looking for protocol for beta 1,6-glucanase enzyme if there any body can provide me protocol with details . thanks in advance.
10 November 2016 9,517 4 View
07 August 2016 1,373 1 View
Hello all, I have one question; why I should do MPLC for my reaction and on TLC it showed only one major product?synthesis of iminosugar
31 December 2015 2,638 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...
02 March 2021 2,300 1 View
Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...
28 February 2021 7,882 2 View
When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...
21 February 2021 3,198 2 View
Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...
19 February 2021 8,351 2 View
Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!
09 February 2021 6,202 1 View
i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?
07 February 2021 5,892 1 View
I am interested in using TLC to detect non-linear xylooligosaccharides. What would be the best visualisation solvent? Would aniline phthalate solution be suitable? I have also seen people...
02 February 2021 7,748 3 View
I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...
28 January 2021 3,867 1 View
i want to do thresholding by segregation of water areas from non water areas for that i am using model maker in ERDAS but i am unable to get the threshold limit value of my image.
26 January 2021 8,277 1 View