The idea is to use rodents and to induce HGT within their microbiomes, one high exposure group, one low exposure and one control. The extent of HGT will then be assessed. What size would my sample have to be at a confidence interval of 95% to indicate a statistical significant difference/correlation. Seeing as my actual samples would be the stool samples collected and the quantified microbiome rather than the actual rodent itself. The rodents are in actual fact vessel that house the actual "study sample".
I think you should rephrase your question in more general terms. You seem to be in a context of ANOVA designs and you are interested in optimal sample size determination. This is covered in most statistical textbooks. There is however no straight answer to your question since it depends primarily on the variability of the data. One way to assess it is to use a pilot survey.
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