Smart VacPrep, anyone had problems with degassing?

12 October 2020 1,907 2 View

Anyone know an effecient method for transfection / siRNA knockdown in organ culture (retina)?

Hello everyone, I have made a nice protocol for culturing whole adult rat retinas in vitro. This is also called organ cultures or whole mount cultures. The retinas are sitting on a filter in a...

15 July 2020 5,300 1 View

Factors affecting precipitation of serum proteins?

Hello, In most modern protocols for metabolomics/lipidomics proteins are precipitated using solvents such as methanol, acetonitrile or isopropanol. After vortexing/mixing the next step is often...

28 April 2020 3,383 2 View

Protocol for FTIR analysis of activated carbons?

Want to analyse functional groups on activated carbons (biomass based). Is there a good protocol including sample preparation and instrumental setup?

02 March 2020 6,972 2 View

Personality. Enherited or socially constructed?

What is your take?

28 July 2019 7,443 17 View

DNA barcoding: is there a COI alignment that spans the Eukaryota?

Regarding the DNA barcode COI (cytochrome oxidase subunit 1), I'm looking for a multiple sequence alignment that, ideally, spans the Eukaryota. Is there any such alignment available for...

30 September 2017 506 5 View

How can you get rid of air bubbles (or blisters) on butyl compound used for inner tubes?

Looking for a method to eliminate air bubbles on butyl inner tube? there are blisters on cured butyl inner tubes, is this a compounding problem or machinery process?

16 September 2016 5,717 2 View

Should I use "locus", "gene", or "marker"?

20 March 2014 8,234 4 View

International business, further globalisation or backlash?

In the field of international business the trend has been a move towards globalisation and integration. However research has shown that lately most government policies have been of a protectionist...

01 January 1970 8,882 59 View

Ambivalence: Where to draw the line between the pathological and the non pathological

When it comes to Ambivalence, it can be a symptom of mental illness but it is also something we experience in our everyday life ( I certainly do, and my wife hates it) . Where would you draw the...

01 January 1970 6,670 9 View

Handheld Portable Raman spectroscopy systems - recommendations and experience?

Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...

07 February 2021 9,691 3 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

Could anyone professional help me to solve this problem?

I have installed the software BRUKER Flexanalysis 3.4 on my computer successfully. But when I tried to import the MS data produced by BRUKER MALDI-TOF-MS, I met with an error described in the...

10 November 2020 8,205 1 View

No title

07 November 2020 6,943 4 View

How to set up the parameters on an NMR instrument to run deuterated sulfuric acid as a solvent?

I have a compound which is only stable in concentrated sulfuric acid and I'd like to get a NMR spectrum so I can deduce the structure. I use a 300 MHz Bruker instrument and the software is topspin.

25 September 2020 5,218 3 View

How can I improve the rietveld refinement model and lower the R bragg factor and chi-square?

I measured a fluconazole drug sample and found 2 phases. Then, for the first time, I tried to apply the rietveld refinement method using Match! and got relatively high values for both R bragg...

27 July 2020 9,492 4 View

How can assessment of performance be improved in Competency-Based Training (CBT)?

Assessment is often cited as the weakest link of Competency-Based Education (CBE). What all can improve the assessment metrics as assessment potentially drives training...

06 July 2020 5,360 21 View

The shoulder on the XRD peak. What could it be?

Dear RG community! I am obtaining very weird peaks on the XRD pattern. There is a shoulder on the left side of the peak looking like a step. Arrow on the picture. It is about 2-3% of the Ka1 peak...

25 June 2020 8,500 12 View

Can discretization method change non-observable system to became an observable one?

Is it theoretically possible, that after discretization by using Talyor Series Expansion, a non-observable nonlinear system will became an observable? It was proved, that used continuous model of...

15 May 2020 3,608 5 View

Looking for guidance to get 3-phase battery dc/ac bi-directional converter simulink circuit?

I'm currently working on peak shaving using Solar PV-battery system via matlab simulink. I'm looking for guidance me to get 3-phase battery dc/ac bi-directional converter simulink circuit....

27 April 2020 1,836 3 View