EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Are there any public datasets of consumer complaints about service industries at the European level?

06 January 2021 1,718 2 View

No title

16 December 2020 9,657 5 View

Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

Best methodology to calculate food footprint in a university campus through students inquiring?

Hello, Considering sustainability and environment negative impacts of animal based products, I would like to calculate food footprint in a Portuguese university campus through students...

05 December 2020 9,098 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Can some one help me to find shear stress and normal across middle of the interface along lap length??

i modeled single lap joint using COMSOL Multiphysics software. i wanted to find shear and peel(normal) distribution across middle of the overlap length pls check the image attached

19 January 2021 6,845 3 View

As the extensive research is going on the dissimilar joining of Mg-Ti alloys, what are the exact applications of Mg-Ti joints?

I have extensive literature review related it, but it ends up in automotive, aerospace and energy sector applications, but didn't provided exact applications of Mg-Ti metals joined by welding

28 December 2020 6,621 1 View

For a joint research work, is there any one provide revenue and operating expenditure data on global airline industry for the past two years?

An invitation for a joint research work

22 December 2020 3,720 1 View

Anyone interested in joint work on writing a review article?

I a writing a review article on cancer work through Mathematical modeling. If anyone interested in this work, kindly let me know

13 December 2020 7,585 10 View

FEM modeling of Joints/ Connection?

The attached Joint.png showed and example case of a joint (2 continuum members are connected). There are 3 characteristics of this, 1. There is a Gap: Cause small amount of rigid body rotation and...

07 December 2020 6,603 3 View

Which automatic data processing for angle detection in Plugin Gait (VICON) do you use?

I would like to analyze gait cycles (running) - is there a smart way for cycle detection and calculation of the angles for each joint (average/maximum) for a certain amount of cycles - without...

07 December 2020 2,586 2 View

What software/app can I use to generate annotations for localizing hand joints in an RGB image?

I wanted to know if there is any software/app that helps to easily annotate hand joints in an RGB image manually.

28 October 2020 9,693 3 View

What is your experience with coated Total Knee Arthroplasty (TKA)?

We recently analyzed coated Total Knee Arthroplasty (TKA) in the German Arthroplasty Registry for periprosthetic joint infection (PJI). We could not show any advantage of coating in terms of PJI....

02 October 2020 4,107 3 View