Bonding, Antibonding or Non-bonding of dz2 orbitals and the bond type

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How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

Failure to deliver mRNA to T cells via LNP, how to solve this problem?

When we used LNP to deliver GFP mRNA to Jurkat cells, the efficiency was as high as 90%. However, when we delivered GFP mRNA to T cells, we failed, with an efficiency of 0.5-0.6%. Could anyone...

06 March 2024 3,341 2 View

How to add 95% confidence ellipse to XYZ scatterplot?

Preferably the effect in the attached file can be achieved.

10 October 2023 5,661 1 View

The slow growth of H9C2 cells and the formation of cell clumps after centrifugation and resuspension might be caused by what factors?

20 August 2023 114 0 View

How to analyze the ferroelectric domains in HAADF image of SrTiO3 films?

Dear All, I got a HAADF image of strained SrTiO3 films with ferroelectricity detected by PFM. Now I want to analyze the ferroelectric domains in the HAADF image. Can anyone tell (or share if...

09 July 2023 5,619 0 View

Question on correlation analyses if the values of some independent variables are 0 and how to compare group LT50 with replicate or population means?

We assessed the ability of 50 age-matched insect lines/populations from 30 diverse geographic regions to survive infection. Median lethal times (LT50) were monitored using five replicates (20-30...

06 March 2023 913 0 View

Can the discriminator in Auxiliary Classifier Generative Adversarial Networks(ACGAN's) be used for multi-classification ?

ACGAN can generate the corresponding target image according to the tag, and its discriminator acts as the tag identification and authenticity judgment in this process. Can a good ACGAN...

09 November 2022 2,556 2 View

What might be causing the mRNA level of the target gene of a cre+/+::flx/flx mouse to decrease 90% but the protein level dosen't change?

I have used the Cdh16-cre mice hybridizing with the X gene-flox mice to generated a tubular epithelial cells-specific X gene deletion mice. There was no problem with the genotyping, the cre was...

04 September 2022 7,304 0 View

What is the oxidized condition of P3HT?

P3HT is often used as a hole transport layer in perovsite solar cell. Is the P3HT need be oxidized as a hole trasnport layer in perovskite solar cell？And what is the oxidized condition of P3HT. Is...

08 December 2021 4,632 3 View

How to prepare a certain concentration of ammonia?

In electrocatalytic ammoxidation, the electrolyte is usually 0.1m NH3+ 1M KOH. How is the concentration of NH3 achieved here？

21 November 2021 3,035 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Can't spin-orbit coupling(SOC) be included in optical properties calculation in CASTEP?

I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...

02 August 2024 2,890 0 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View