Hi all,
Recently I've been setting up Doric's ilFMC4 (3rd gen) cube for fiber photometry experiments. It says that in the cube itself there is an isobestic excitation (IE) port, a regular excitation (E) port of our wavelength needed, and a fluorescence (F) port that has a built in detector and amplifier. I had wired up the cube to a NI DAQ and everything was working fine two days ago. I removed the BNC cables connecting the E and F ports to the DAQ in order to use an oscilloscope to test for AC/DC current (maybe not the best idea?).
When I reconnected them and tested the system, I found that I had no light coming out of the the fiber optic patch cord that is connected to my sample port, where it's supposed to shine from. Usually I get about 40-50uW from the end of that cable that then goes into a fiber optic rotary joint (at this point I will get about 30-40uW) that is then used to shine light onto a mouse's implanted fiber (this final output has about 25-30uW of light at max). I could not get light to come out of even the first step, where it would usually be about 40-50uW.
I maxed out the LEDs to see if I got anything, and it looks like only the IE might be on. I saw absolutely no blue light at all. Any ideas what could have gone wrong? The cube was turned off between two days ago and when I switched it on today, and the only thing I fiddled around with was the E and F BNC cables to attempt to measure voltage on the oscilloscope. It runs on a power cord, not battery.