Bioinformatics tools to predict potential ligand?

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Publishing in F1000Research??

Hi, Anyone has first hand experience of publishing in F1000Research. I guess it follows open peer review and does not encourage measurable parameters like impact factor! What are pros and cons...

05 June 2019 9,763 1 View

Protein-protein interaction??

Hi, I have two proteins that are his tagged at N-terminus. One of them forms hexamer around 370kDa and other is 17kDa. I want to check their interaction and get Kd value. Which of the following...

03 April 2019 1,604 5 View

Designing mutation experiments??

Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...

02 March 2019 4,959 5 View

E.Coli expression strain for expressing mycobacterial membrane protein gene?

Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...

11 December 2018 1,979 3 View

Bioinformatic tool to predict flexible region in a protein?

Hi, Can anyone please tell some bioinformatics server that helps in predicting the location of flexible region in a protein.?

10 November 2018 6,649 0 View

Difference between Coomassie Brilliant Blue R-250 and G-250??

Hi, Can anyone please highlight the difference between Coomassie Brilliant Blue R-250 and G-250?? Which one of them will stain the SDS gel faster?

09 October 2018 3,062 0 View

Citing preprint articles?

Hi, I was just curious to know whether the scientific articles and review published in preprint repository like bioRxiv are citable? If yes, is it good practice to do that since mostly such...

09 October 2018 4,812 4 View

How to degass a buffer with detergent?

Hi, I want to add about 5 CMC DDM detergent in my Size exclusion buffer. Is it advisable to add detergent and then degass by vacuum or degass the buffer first and then add DDM dropwise slowly??

08 September 2018 4,241 3 View

Publishing in bioRxiv??

Hi, Can someone please tell pros and cons briefly of publishing in preprint servers like bioRxiv?

07 August 2018 7,726 1 View

1M NaOH cleaning overnight?

Hi, One of my colleague put the Superdex 200 10/300 GL column mistakenly in 1M NaOH at flowrate of 0.2ml/min overnight instead of water wash. Will the column be stable? How to know that the...

07 August 2018 5,344 1 View

Which software tools are best for enhancing diagnostic accuracy in chest X-ray imaging using image reconstruction and neural networks?

I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...

22 July 2024 3,794 1 View

I want to conduct a research using quasi experimental design to test two treatment and one control what type of statistical tools do l use ?

What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks

18 July 2024 3,581 3 View

Which research tool for expert validation for our study?

I am a 3rd year Computer Science student currently writing our Bachelor's thesis about finding diverse k-shortest paths in pedestrian networks. We have chosen 3 local areas as our proposed...

15 July 2024 4,289 0 View

Figure plot in scientific articles?

Hi all, Do you know how to plot this figure? I found many of them in Nature journals but have not figured out how to make them. Which software/tool do you suggest? Thank you very much.

15 July 2024 8,384 2 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

What is the difference between OTU and ASV analysis?

For microbiome data analysis

13 July 2024 4,542 2 View

How to predict Shine-Dalgarno sequence ?

Hello, I am Master student under molecular biology. I want to find the Shine-Dalgarno sequence after performing a BLAST search in TargetRNA2. Could you please suggest a web tool to predict the...

07 July 2024 9,598 1 View

Can the meaning of STATISTICS as thought by me be accepted?

Meaning of the term STATISTICS as I have thought of is as follows: S T A T I S T I C S Systematic Tentative Analytical...

28 June 2024 4,011 1 View

How can I convert remote sensing (RS) images to vector data automatically?

I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...

22 June 2024 6,165 2 View

Should a researcher seek permission from the author of a research instrument accessed online?

I found this online research tool which is relevant to my research project. Is it safe to use it without the author's consent or should I seek first his permission before using the instrument?

19 June 2024 603 0 View