Hello,

Question: Could someone tell me a bioinformatic program to identify pre-design shRNA targets within nucleotide sequence of other species ? or

Could someone tell me a bioinformatic program to identify the different elements of a commercial shRNA?

Example: Online there is shRNA for mouse and KLF4. I would like to know if I might use that shRNA for dogs into same gene.

Problem: like shRNA has different elements--> linker, sense sequence, loop, anti sense sequence. I will never have an clean alignment (global or local).

Advices are welcome

Thank you so much