Jonathan Wagner

3 Questions 13 Answers 0 Followers

Questions related from Jonathan Wagner

Why can't I get colonies when transforming with a new plasmid?

I often order genes synthesized and subcloned into pET28a at Genscript. Last time around I also ordered one subcloned into pET26b in order to add a pelB peptide sequence. Unfortunately, when I got...

22 January 2025 4,425 5 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

13 June 2024 7,445 1 View

Small scale columns for immobilized enzyme activity screening?

I have been screening the activity of immobilized enzymes in shaken tubes and need to move into testing activity under flow in a column. I am looking for recommendations on what column / pump /...

23 November 2019 6,664 1 View