I'm looking for a decent method to ship multiple strains of yeast on filter paper (around 85 mm in size) for shipping using liquid culture.
I'm having little luck reviving yeast strains blotted out onto paper, even after waiting 3 days at 30 degrees C for incubation. Does anyone have a reliable method for pipetting a mixture of yeast culture, media and preservative (such as glycerol) onto paper? Soaking an entire piece of filter paper into the yeast culture is unfortunately not an option as I need to do multiple strains on one piece of paper.
Streaking colonies onto wet media isn't a good option for me either as I'm looking to try and send large amounts of filters out and that might be too time consuming with not enough colonies to place, though I am thinking of it as a last resort.
Also, will using E. Coli Filter Paper blot techniques be equivalent?
Any help would be greatly appreciated, thanks.
Hi there,
I think the following should be very helpful:
https://suigenerisbrewing.blogspot.com/2013/04/mailing-yeast.html
Yup, the description above is very close to what Ive used pretty sucessfully. You can also use the same basic procedure, but simply dip the sterile filter paper into a streak of cells on solid medium. Keeping in the fridge for a day or two will help the yeast survive. I havent tried this, but if you have a plate where colonies that are sufficiently far from each other can be blotted onto the same piece of paper, you could ship many strains on one piece.
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