I am working on separating monosaccharides on TLC silicagel 60G. The monosaccharides I would like to resolve include: N-acetyl-D-glucosamine, L-Fucose, L+Arabinose, D-Glucuronic Acid, D+Galactose, D+Glucose, D+Mannose, D-Arabinose, D-Xylose, D+Galacturonic acid, L-Rhamnose, D-Ribose, and several oligosaccharides including D+Maltose, D+Tetraose, D+Maltohexose, D+Maltoheptaose, D+Isomaltose, Maltopentaose and Maltotriose. I am aware of several methods capable of separating most of these, including EPAW (ethylacetate:pyridine:acetic acid:water) and BAW (butanol:acetic acid:water). I haven't had good resolution for some of the monosaccharides and will try to do it again, but in the mean time would like to see if anyone has tried a better system, i.e. plates with different binding polymers, different solvent system, tips and tricks, etc. I am aware that GC-MS might be the way to go, but given a low concentration and the visibility provided by TLC I would like to spend time on trouble-shooting TLC method first.