I'm planning to do some RNA-seq experiments on Enterobacteriaceae (specifically carbapenem-resistant Klebsiella pneumoniae isolates) and would greatly appreciate any advice about kits or protocols (+/- Trizol) people have had luck with. I know that a bead-beating step is sometimes used but I'm not sure if this will be necessary so would appreciate any advice on that.
Thank you very much!
Thank you so much, Kunal! Do you use Trizol with the PureLink kit?
See the link for answer
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1726-3
pl see the ans. in link
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1726-3
I've used http://www.bio-rad.com/en-cn/product/aurum-total-rna-mini-kit and http://www.zymoresearch.com/rna/rna-isolation/rna-from-trizol-tri-reagent/direct-zol-rna-miniprep with H. pylori. The Zymo kit will pick up small RNAs as well but I don't think the Bio-Rad one does
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