Hello,
We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments.
This time, we need to make a PLT technique with K4M or LR white resin. Our results were bad as the ultrastructure was poorly defined.
Do you a protocol with the buffers, times and temperatures, whiche allow us to have good immunogold signal and a not so bad ultrastructure?
Thank you in advance
Brent E Gowen
Consider going into Lowicryl HM20 with the PLT technique. I usually polymerize the HM20 below 4 degrees C which results in good labelling with adequate morphology. I've used both HM20 and LR White (and LR Gold) on the same samples. HM20 sections always look better morphologically.
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