I want to buy a perfusion system for a confocal microscope. Would you recommend one? Mostly, I want to do calcium imaging. I would like one that you can control the flow rate. Thank you so much for the advice!
Do you have any special need for these calcium imaging experiments? I do confocal or Bi-photon imaging on a daily basis, and all the rigs I could see used standards electrophysiology perfusion systems. They are mainly DIY, with 1 or 2 peristaltic pumps and a manual flow regulator. It is really cheap and easy to do with Tygon tubes and some fittings. You can add a temperature controller of course.
The LEGO device Jonas mentionned is mainly for cell cultures, I am not certain it could be suitable for thicker tissue (like slices) where you need a higher flow rate. And it is perfectly suited for complex sequences of rinsing / Solution change, etc... but might be overkill if you only want to record your cells in standard ACSF with only 1 or 2 solution change.