I am hoping to obtain some guidance regarding the best method to store mushroom fruiting bodies from the field for DNA preservation and subsequent Illumina sequencing.
From what I have read already my main options are:
1) Drying - recommended below 60 degrees.
Article Comparison of Different Drying Methods for Recovery of Mushroom DNA
Article Assessment of the DNA Quality in Mushroom Specimens:Effect o...
2) Immediate freezing - I have seen this range from immediate freezing at - 80, to -4.
Article Finding flies in the mushroom soup: Host specificity of fung...
3) Storage in ethanol.
I am leaning towards drying as a preservation method. Does anyone have any advice for/against any of these methods? There are a number of papers that explore various drying temperatures etc, but I am yet to see a comparison between storing in liquid buffer versus drying. I wish to barcode the ITS region for simple fungal identification, followed by CO1 sequencing to explore the associated invertebrate community.
Thank you in advance,
Kirsty.
Depends on the size, but I prefer using dried samples. If you have small size fruiting bodies, I would dry them in one piece, if medium sized I would cut them half and dry the helves, if big sized, I would make thick slices (about 1 cm thick each) and dry them in this way.
Drying the specimens as Attila Fodi says is a good option but it is very important to dry them at 40 ºC in temperature or less
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