Dear all,
I have a datasheet reporting hormone concentrations in two different A. thaliana genotypes, grown under three different treatments. Each treatment has 5 technical replicates.
Does the two-way Anova is the best way to analyse these data?
I need to install this program
25 July 2024 4,756 0 View
Following https://catniplab.github.io/Tafazoli-LearningControlBrain/ and https://insights.princeton.edu/2020/05/learning-to-control-the-brain/ which commercial technology is available ? Probably...
15 July 2024 4,339 0 View
I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...
30 June 2024 4,107 4 View
I'been seeing those white spots at the end of my wet-transfer lately. Since the Ponceu Red doesn't stain I guess there isn't any protein. Since it's under the 42kDa mark It isn't a problem since I...
18 June 2024 1,734 0 View
Hello, Can somebody please give me some reference to a paper or book where it is explained how to condensate: * from a Q8 serendipity FE to a Q4 FE with drilling DOFs (16 DOFs to 12 DOFs). *...
05 June 2024 936 1 View
Organoid with Claustro can be linked to LLM Neuroplatform - FinalSpark , finalspark.com/neuroplatform/ can be linked to chatGPT ? What to do with this ? Avatar Pilots ? Studying the case of...
28 May 2024 3,302 0 View
Hi everyone, I am culturing colorectal cancer organoids (Control and Experimental) and I observe a faster growing (number and size of organoids) in experimental condition. Now the question is,...
27 May 2024 9,258 0 View
The truth is that I have had many, many problems to be able to see a specific band of this protein. My problems have ranged from bad transfers, to unknowns that I have not been able to answer. The...
26 April 2024 8,595 4 View
Hello I have to work with a preform analyzer model 2650 - Photon Kinetics, but I have no preforms yet to start the calibration and the tests. Is it possible use other kind of sample with a well...
25 April 2024 564 1 View
Although I can detect the protein (above 200), leaving it for longer in the transfer (plus 2h), I think I am losing my endogenous protein, as its detection is not getting good. How can I solve it?
07 April 2024 2,338 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Would anyone be able to advise me... I have an RCT with a control and experimental group. Participants were recruited from one school (n=59). Participants were assessed using repeated measures on...
04 August 2024 4,040 6 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...
24 July 2024 3,284 6 View
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View