Best customized array to run miRNA expression in human blood?

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Can some indicate a technique - or approach - to measure the thickness of a 20µm film and thickness with water uptake?

Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...

08 July 2024 4,799 4 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Is there any situation where the use of negative q exponents in the formula of Hill numbers is justifiable?

Hill numbers have become an elegant and powerful unifying tool in the measurement of many levels of biological diversity. Although mathematically possible, the use of negative values for the...

09 June 2024 9,302 2 View

Is there any news on the treatment of macular injury from high-intensity LED light?

Hi everyone, I'm looking for papers regarding macular injury from LED light, but it seems there's only publications talking about acute lesions. Thanks in advance. Cheers!

13 May 2024 7,160 0 View

How can metals and polymers be separated quickly in a flexible pipe used in the oil and gas industry?

In subsea decommissioning projects, the time it takes to separate materials from a flexible pipe for recycling is important for the quality of the operation. So, what machines and strategies can...

09 April 2024 3,241 0 View

What other point georeferencing clustering model, besides k-means, would you recommend I use for a dataset above 10,000 points?

We have a dataset containing over 10,000 georeferenced points spread throughout our municipality, each representing a user with specific demands. Our goal is to dynamically group these users based...

08 April 2024 9,909 0 View

Can I purify large quantities of proteins using the ÄKTA protein purification system equipped with size exclusion or ion exchange columns?

Can I purify large quantities of phycocyanin (current concentration of 30%) using the ÄKTA system equipped with size exclusion or ion exchange columns? Could I perform multiple injections to...

26 March 2024 5,031 2 View

What is the most suitable conductivity cell (k=0.1; K=1) for evaluating the conductivity of pharmaceutical formulations such as liposomes, LNPs...?

Dear, What is the most suitable conductivity cell (k=0.1; K=1) for evaluating the conductivity of pharmaceutical formulations such as liposomes, LNPs...

24 March 2024 5,466 2 View

Barlat 1991 model. How are the parametes calculated?

I am doing a comparison between different anisotropy models. Now, I am understanding the Barlat 1991 model. I understand the concept but I have some doubts about how to proceed to calculate the...

10 March 2024 3,354 1 View

What are the best websites to find academic events?

I'm from Brazil, but I want to start proposing communications and papers for international events. Are there any specific websites I can use to find events? I really appreciate any help you can...

06 February 2024 2,489 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View