In the isolation of Vibrio from water sample, besides using TCBS what other selective media can be used
There are quite a number of other selective media that can be used. Cellobiose-polymyxin B-colistin agar can be used for Vibrio cholera and V. vulnificus and does indeed perform better than TCBS for them. HiCrome Vibrio Agar is a chromogenic agar from Fluka and help select for Vibrio in the presence of sucrose fermenters. There is also Glucose Salt Teepol Broth from Fluka. Good luck in your isolations.
this pdf from CDC could help you
http://www.cdc.gov/cholera/pdf/Laboratory-Methods-for-the-Diagnosis-of-Vibrio-cholerae-chapter-4.pdf
http://www.chromagar.com/food-water-chromagar-vibrio-focus-on-vibrio-species-23.html#.VidsYsufpwQ
check this reference out it describes a special method called the triple plating method which is equal to PCR in accuracy but it was done with vibrio vulnificus specifically:
- Williams TC, Froelich B, Oliver JD. A new culture-based method for the improved identification of Vibrio vulnificus from environmental samples, reducing the need for molecular confirmation. J. Microbiol. Methods. 2013 Jun;93(3):277–83.
Cellobiose-polymyxin B-colistin agar can be used for Vibrio cholera and V. vulnificus and does indeed perform better than TCBS for them. HiCrome Vibrio Agar is a chromogenic agar from Fluka and help select for Vibrio in the presence of sucrose fermenters.
If an enrichment procedure is desired to enhance isolation of vibrios, alkaline peptone water with 1% NaCl (pH 8.5) can be inoculated (at least 20mL) and incubated for 5 - 8 hours at 35°C before subculturing to TCBS. CHROMagar Vibrio (CHROMagar Microbiology, Paris) for the isolation and identification of V. cholera, V. parahemolyticus and
V. vulnificus.
Source: Mahon book
CHROMagar Vibrio(CV) is the most suitable medium for the isolation of V. parahaemolyticus from seafood and environmental samples.Sodium Taurocholate broth (ST) broth is superior compared to Alkaline Peptone Water (APW) broth and allowed better isolation of V. parahaemolyticus. ST broth-CV agar combination is recommended for the isolation of V. parahaemolyticus from seafood and environmental samples.
Source: Minimol, V.A., Balange, A.K., Nayak, B.B. and Kumar, S., 2018. Distribution of potentially pathogenic Vibrio parahaemolyticus in seafood and the aquatic environment in Mumabai, India.
Thank you @ Sambit Kisore Das. Very informative. I can't agree less.
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